Composite

Part:BBa_K2578810:Design

Designed by: Yam Shing Fung   Group: iGEM18_Hong_Kong_JSS   (2018-09-14)


Protein coding sequence for Homo sapiens metallothionein 1 (MT1A)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 865
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 62


Design Notes

no expression


Source

NCBI

References

[1] https://www.ncbi.nlm.nih.gov/gene/4489

[2] https://parts.igem.org/Part:BBa_J23100

[3] https://parts.igem.org/Part:BBa_B0032

[4] https://parts.igem.org/Part:BBa_B0015

[5] N Thirumoorthy, KT Manisenthil Kumar, A Shyam Sundar, L Panayappan, Malay Chatterjee (2007). Metallothionein: An overview. World journal of gastroenterology, ISSN 1007-9327

[6] Almaguer-Cantú V1, Morales-Ramos LH, Balderas-Rentería I. (2011) Biosorption of lead (II) and cadmium (II) using Escherichia coli genetically engineered with mice metallothionein I. Water Sci Technol. 2011;63(8):1607-13.

[7] Dziegiel, P., Pula, B., Kobierzycki, C., Stasiolek, M., Podhorska-Okolow, M. (2016), Metallothioneins in Normal and Cancer Cells, Springer International Publishing, DOI: 10.1007/978-3-319-27472-0

[8] Dziegiel, P., Pula, B., Kobierzycki, C., Stasiolek, M., Podhorska-Okolow, M. (2016), Metallothioneins in Normal and Cancer Cells, Springer International Publishing, DOI: 10.1007/978-3-319-27472-0

[9] M Karin and R I Richards (1984). The human metallothionein gene family: structure and expression. Environ Health Perspect. 1984 Mar; 54: 111–115.

[10] Scheller JS, Irvine GW, Wong DL, Hartwig A, Stillman MJ.(2017) Stepwise copper(i) binding to metallothionein: a mixed cooperative and non-cooperative mechanism for all 20 copper ions. Metallomics. 2017 May 24;9(5):447-462. doi: 10.1039/c7mt00041c.